VOL 67_2016/068
SPE-RRLC DETERMINATION OF SOME PESTICIDE RESIDUES IN APPLE JUICE
L. Velkoska-Markovska*, B. Petanovska-Ilievska, I. Stojanovska, M. S. Jankulovska
Faculty of Agricultural Sciences and food, “Ss. Cyril and Methodius” University, Skopje, Republic of Macedonia
*corresponding author: levemar@gmail.com; lencevm@zf.ukim.edu.mk
Abstract
This paper presents the development of RRLC (Rapid Resolution Liquid Chromatography) method for simultaneous determination of pesticides methomyl, methidathion and propiconazole in different clear apple juice samples. The experiments are performed using rapid resolution liquid chromatography system coupled with UV-VIS diode array detector. The developed high speed reversed-phase (RP) liquid chromatography method is carried out on the Purospher® Star RP-18 endcapped (30 mm × 4 mm; 3 μm) column, mobile phase consists of acetonitrille and water (50/50, V/V), flow rate of 1 mL/min, column temperature at 25 ºC and UV detection at 220 nm and 235 nm. Prior to RRLC analysis, the samples are cleaned up and concentrated using a solid-phase extraction (SPE). To assess the validity of the developed method, the following parameters are examined: selectivity, linearity, repeatability (precision), limit of detection, limit of quantification and accuracy.
Key words: RRLC determination, SPE, UV-DAD, pesticide residues, apple juice
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