VOL 67_2016/076
QUANTITATIVE DETERMINATION OF 2,4-D IN PESTICIDES MONOSAN HERBI AND DMA-6
1Faculty of Agricultural Sciences and food, “Ss. Cyril and Methodius” University in Skopje, Republic of Macedonia
*corresponding author:levemar@gmail.com, lencevm@zf.ukim.edu.mk
Abstract
A rapid and reliable method for determination of active ingredient 2,4-D ((2,4-dichlorophenoxy)acetic acid) in the pesticide formulations Monosan herbi and DMA-6 is presented. The procedure utilizes high-performance liquid chromatography (HPLC) followed by UV diode array detection and two analytical columns with different stationary phases and dimensions. The better results for identification and quantitation of the active ingredient in two pesticides are achieved using LiChrospher 60 RP-select B (250 x 4 mm, 5 µm) column, UV detection at 220 nm, temperature at 25 0C, mobile phase consisted of acetonitrile and water (60/40; V/V) and flow rate of 1 mL/min. The method is validated by testing linearity, precision, recovery, LOD and LOQ. The values for multiple correlation coefficient (R2 > 0.999), relative standard deviation (RSD) of retention time and peak area (RSD ≤ 1.18 %), recoveries ranged from 98.16 % - 101.38 %, with RSD of 0.10 % - 1.96 %, revealed that the developed method has a good linearity, precision and accuracy. The proposed method is applicable for fast and accurate determination of active ingredient 2,4-D in the pesticides Monosan herbi and DMA-6.
Key words: HPLC-method, UV-detection, 2,4-D, Monosan herbi, DMA-6.
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